Comparing FemtoGlow and AttoGlow Substrates
Choosing between FemtoGlow HRP substrates and AttoGlow AP substrates depends on your specific Western blotting needs. This comparison guide helps you select the optimal detection system for your research.
FemtoGlow HRP Substrates
Best For:
- Standard Western blotting protocols
- High-throughput screening
- Budget-conscious laboratories
- ELISA and chemiluminescent immunoassays
Advantages:
- Fast signal development (1-5 minutes)
- Compatible with all standard HRP-conjugated antibodies
- Economical for routine applications
- Available in single-bottle or two-bottle formats
Sensitivity Range:
Femtogram to picogram detection depending on formulation
AttoGlow AP Substrates
Best For:
- Ultra-low abundance proteins (attogram detection)
- Multiplexing experiments
- Membrane stripping and reprobing
- Applications requiring extended signal stability
Advantages:
- Attogram-level sensitivity with enhancer
- Extended signal plateau (2+ hours)
- No endogenous peroxidase interference
- Available in 450nm and 540nm emission variants
Sensitivity Range:
Attogram to femtogram detection with enhancer reagents
Side-by-Side Comparison
| Feature | FemtoGlow (HRP) | AttoGlow (AP) |
|---|---|---|
| Sensitivity | Femtogram | Attogram |
| Signal Duration | 30 min – 2 hours | 2+ hours |
| Development Time | 1-5 minutes | 25-45 minutes |
| Price Point | Economy | Premium |
Making Your Choice
Choose FemtoGlow if: You need fast, reliable detection for medium-to-high abundance proteins with standard HRP workflows
Choose AttoGlow if: You require maximum sensitivity for rare proteins or plan to strip and reprobe membranes
Order Samples
Not sure which substrate is right for you? Contact us to request trial sizes of both FemtoGlow and AttoGlow substrates.